Keywords

Vldl, very low density lipoprotein, lipoprotein, apolipoprotein, apob, apob100, trafficking, cholesterol, hepatocyte, lipid, 2d dige, radiolabel, vtv, pg vtv, pg vtv, post golgi, post golgi vldl transport vesicle

Abstract

Amongst its numerous functions, the liver is responsible for the synthesis and secretion of very low-density lipoprotein (VLDL). VLDL particles play the important role of facilitating the transport of lipids within the aqueous environment of the plasma; yet high plasma concentrations of these particles result in the pathogenesis of atherosclerosis, while low VLDL secretion from the liver results in hepatic steatosis. VLDL synthesis in the hepatocyte is completed in the Golgi apparatus, which serves as the final site of VLDL maturation prior to its secretion to the bloodstream. The mechanism by which VLDL’s targeted transport to the plasma membrane is facilitated has yet to be identified. Our lab has identified this entity. Our findings suggest that upon maturation, VLDL is directed to the plasma membrane through a novel trafficking vesicle, the Post-Golgi VLDL Transport Vesicle (PG-VTV). PG-VTVs containing [3H] radiolabeled VLDL were generated in a cell-free in vitro budding assay for study. First, the fusogenic capabilities of PG-VTVs were established. Vesicles were capable of fusing with the plasma membrane and delivering the VLDL cargo for secretion in a vectorial manner. The next goal of our study is to characterize key regulatory molecular entities necessary for PG-VTV biosynthesis. A detailed analysis was undertaken to determine the PG-VTV proteome via western blot and two-dimensional difference in gel electrophoresis. The identification of key molecular regulators will potentially offer therapeutic targets to control VLDL secretion to the bloodstream.

Notes

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Graduation Date

2013

Semester

Summer

Advisor

Siddiqi, Shadab

Degree

Master of Science (M.S.)

College

College of Medicine

Department

Molecular Biology and Microbiology

Degree Program

Biomedical Sciences; Biomedical Sciences Track

Format

application/pdf

Identifier

CFE0005236

URL

http://purl.fcla.edu/fcla/etd/CFE0005236

Language

English

Release Date

February 2019

Length of Campus-only Access

5 years

Access Status

Masters Thesis (Campus-only Access)

Subjects

Dissertations, Academic -- Medicine, Medicine -- Dissertations, Academic

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