Keywords

high pressure raman spectroscopy, proteins, biomolecules, fluorescence

Abstract

Functional properties of biological molecules and cells are affected by environmental parameters such as temperature and pressure. While Raman spectroscopy provides an intrinsic probe of molecular structural changes, the incorporation of a microscope enables studies of minuscule amounts of biological compounds with spatial resolution on a micron scale. We have developed a novel setup which combines a Raman microscope and a high pressure cell. A micro-capillary made out of fused silica simultaneously serves as the supporting body and the optical window of the pressure cell. The cell has been tested over the pressure range from 0.1 to 4 kbar. Raman spectra of less than 100 nanoliter amount of amino acid and protein solutions have been measured in the micro-capillary high pressure cell. It is also demonstrated that the setup is well suited for spectrally resolved fluorescence measurements at variable pressure.

Notes

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Graduation Date

2007

Semester

Spring

Advisor

Schulte, Alfons

Degree

Master of Science (M.S.)

College

College of Sciences

Department

Physics

Degree Program

Physics

Format

application/pdf

Identifier

CFE0001683

URL

http://purl.fcla.edu/fcla/etd/CFE0001683

Language

English

Release Date

May 2007

Length of Campus-only Access

None

Access Status

Masters Thesis (Open Access)

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