Title
Developing a novel serum-free cell culture model of skeletal muscle differentiation by systematically studying the role of different growth factors in myotube formation
Abbreviated Journal Title
In Vitro Cell. Dev. Biol.-Anim.
Keywords
Muscle differentiation; Muscle-nerve coculture; Myotube; Serum-free; medium; Synthetic substrate; NEUROTROPHIC FACTOR; DEFINED SYSTEM; SILICON MICROSTRUCTURES; EPIGENETIC; CONTROL; MESSENGER-RNAS; RAT; CARDIOTROPHIN-1; RECEPTOR; GDNF; MOTONEURONS; Cell Biology; Developmental Biology
Abstract
This work describes the step-by-step development of a novel, serum-free, in vitro cell culture system resulting in the formation of robust, contracting, multinucleate myotubes from dissociated skeletal muscle cells obtained from the hind limbs of fetal rats. This defined system consisted of a serum-free medium formulation developed by the systematic addition of different growth factors as well as a nonbiological cell growth promoting substrate, N-1[3-(trimethoxysilyl) propyl] diethylenetriamine. Each growth factor in the medium was experimentally evaluated for its effect on myotube formation. The resulting myotubes were evaluated immunocytochemically using embryonic skeletal muscle, specifically the myosin heavy chain antibody. Based upon this analysis, we propose a new skeletal muscle differentiation protocol that reflects the roles of the various growth factors which promote robust myotube formation. Further observation noted that the proposed skeletal muscle differentiation technique also supported muscle-nerve coculture. Immunocytochemical evidence of nerve-muscle coculture has also been documented. Applications for this novel culture system include biocompatibility and skeletal muscle differentiation studies, understanding myopathies, neuromuscular disorders, and skeletal muscle tissue engineering.
Journal Title
In Vitro Cellular & Developmental Biology-Animal
Volume
45
Issue/Number
7
Publication Date
1-1-2009
Document Type
Article
Language
English
First Page
378
Last Page
387
WOS Identifier
ISSN
1071-2690
Recommended Citation
"Developing a novel serum-free cell culture model of skeletal muscle differentiation by systematically studying the role of different growth factors in myotube formation" (2009). Faculty Bibliography 2000s. 1453.
https://stars.library.ucf.edu/facultybib2000/1453
Comments
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