Title

MicroRNA let-7 Regulates 3T3-L1 Adipogenesis

Authors

Authors

T. W. Sun; M. G. Fu; A. L. Bookout; S. A. Kliewer;D. J. Mangelsdorf

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Mol. Endocrinol.

Keywords

FACTOR HMGI-C; ADIPOCYTE DIFFERENTIATION; TRANSGENIC MICE; GENE; EXPRESSION; IDENTIFICATION; REPRESSES; PROMOTER; LIPOMAS; BINDING; Endocrinology & Metabolism

Abstract

Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of events including clonal expansion, growth arrest, and terminal differentiation. The mechanisms coordinating these different steps are not yet fully understood. Here we investigated whether microRNAs (miRNAs) play a role in this process. Microarray analysis was performed to detect miRNA expression during 3T3-L1 preadipocyte differentiation. Several miRNAs, including let-7, were up-regulated during 3T3-L1 adipogenesis. Ectopic introduction of let-7 into 3T3-L1 cells inhibited clonal expansion as well as terminal differentiation. The mRNA encoding high-mobility group AT-hook 2 (HMGA2), a transcription factor that regulates growth and proliferation in other contexts, was inversely correlated with let-7 levels during 3T3-L1 cell adipogenesis, and let-7 markedly reduced HMGA2 concentrations. Knockdown of HMGA2 inhibited 3T3-L1 differentiation. These results suggest that let-7 plays an important role in adipocyte differentiation and that it does so in part by targeting HMGA2, thereby regulating the transition from clonal expansion to terminal differentiation. (Molecular Endocrinology 23: 925-931, 2009)

Journal Title

Molecular Endocrinology

Volume

23

Issue/Number

6

Publication Date

1-1-2009

Document Type

Article

Language

English

First Page

925

Last Page

931

WOS Identifier

WOS:000266403600018

ISSN

0888-8809

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