Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture
Abbreviated Journal Title
adult rat spinal cord neurons; acetylcholine-chloride; electrophysiology; glutamate (N-acetyl-DL-glutamic acid); motoneuron; neurotransmitters; regeneration; serotonin; serum-free medium; silane; substrate; RAT HIPPOCAMPAL-NEURONS; DEFINED SYSTEM; MOTONEURON; GLUTAMATE; BRAIN; GABA; MICRODIALYSIS; ACETYLCHOLINE; TRANSMISSION; SURVIVAL; Neurosciences
The ability to culture functional adult mammalian spinal cord neurons represents an important step in the understanding and treatment of a spectrum of neurological disorders including spinal cord injury. Previously, the limited functional recovery of these cells, as characterized by a diminished ability to initiate action potentials and to exhibit repetitive firing patterns, has arisen as a major impediment to their physiological relevance. In this report, we demonstrate that single temporal doses of the neurotransmitters serotonin, glutamate (N-acetyl-DL-glutamic acid) and acetylcholine-chloride lead to the full electrophysiological functional recovery of adult mammalian spinal cord neurons, when they are cultured under defined serum-free conditions. Approximately 60% of the neurons treated regained their electrophysiological signature, often firing single, double and, most importantly, multiple action potentials. (c) 2007 Elsevier Inc. All rights reserved.
"Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture" (2008). Faculty Bibliography 2000s. 257.