Title

Somatic embryogenesis in the woody legume Calliandra tweedii

Authors

Authors

S. Kumar; V. Agrawal;S. C. Gupta

Comments

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Abbreviated Journal Title

Plant Cell Tissue Organ Cult.

Keywords

in vitro regeneration; tree tissue culture; CERCIS-CANADENSIS; REGENERATION; EMBRYOS; INVITRO; TREE; Biotechnology & Applied Microbiology; Plant Sciences

Abstract

In vitro somatic embryogenesis was established from 1-1.2 cm long internodal and petiolar segments excised from 20-year-old woody legume, Calliandra tweedii Benth trees. Within 6-7 weeks, globular, heart, torpedo-shaped and dicot embryos differentiated directly. Among the cytokinins and auxins tested, 1 muM 2iP in Murashige and Skoog's (MS) medium induced well-organized normal embryos in 71% of the internodal segments. Petiolar explants responded best on 0.5 muM NAA wherein 30% of the cultures developed normal embryos. Somatic embryos developed roots and shoots when they were retained for 90 days in old desiccated MS medium. Plants ready for transfer to soil were regenerated through somatic embryogenesis in 4-5 months. These grew well after transfer to soil.

Journal Title

Plant Cell Tissue and Organ Culture

Volume

71

Issue/Number

1

Publication Date

1-1-2002

Document Type

Article

Language

English

First Page

77

Last Page

80

WOS Identifier

WOS:000177102800010

ISSN

0167-6857

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