Title

Biochemical function of msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv: Biosynthesis of monomethyl branched unsaturated fatty acids

Authors

Authors

V. S. Dubey; T. D. Sirakova; M. H. Cynamon;P. E. Kolattukudy

Abbreviated Journal Title

J. Bacteriol.

Keywords

DIMYCOCEROSYL PHTHIOCEROL SYNTHESIS; BACILLUS-CALMETTE-GUERIN; MULTIFUNCTIONAL ENZYME; GENE-CLUSTER; BOVIS BCG; SYNTHASE; FORTUITUM; FAMILY; DISRUPTION; COMPLEX; Microbiology

Abstract

We show that the disruption of one of the mycocerosic acid synthase (mas)-like genes, msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv generates a mutant incapable of producing monomethyl branched unsaturated C-16 to C-20 fatty acids that are minor constituents of acyltrehaloses and sulfolipids. The msl5 mutation did not cause any significant change in the acyl lipid composition and also did not affect growth in culture, in mouse alveolar macrophage cell line MH-S, or in the murine lung.

Journal Title

Journal of Bacteriology

Volume

185

Issue/Number

15

Publication Date

1-1-2003

Document Type

Article

Language

English

First Page

4620

Last Page

4625

WOS Identifier

WOS:000184237800039

ISSN

0021-9193

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