Lysozyme levels in the nasal secretions of patients with perennial allergic rhinitis and recurrent sinusitis
Abbreviated Journal Title
Ann. Allergy Asthma Immunol.
MAJOR BASIC-PROTEIN; ANTIMICROBIAL PEPTIDE; TRACHEAL EPITHELIUM; HOST; DEFENSE; LAVAGE FLUID; EOSINOPHIL; LACTOFERRIN; CHILDREN; MUCOSA; ASTHMA; Allergy; Immunology
Background: The association of perennial allergic rhinitis (PAR) with recurrent sinusitis (RS) is well recognized. Anatomic abnormalities at the osteomeatal complex or ciliary dysfunction may play a significant role in some patients. However, for most patients with allergy, the determinants of RS are unknown. Objective: To determine whether altered concentrations of antimicrobial peptides and proteins, such as lysozyme, lactoferrin, human beta-defensin-2 (HBD-2), and human neutrophil peptides 1 to 3 (HNP-1 to 3), contribute to the development of RS in patients with PAR. Methods: Nasal secretions were collected by vacuum aspiration from 15 individuals with PAR+RS, 16 with PAR alone, and 16 controls. Lysozyme and lactoferrin levels were determined in nasal secretions by using quantitative enzyme-linked immunosorbent assay, and HBD-2 and HNP-1 to 3 levels were determined in nasal secretions by using semiquantitative Western blot analysis. Eosinophil-derived neurotoxin (EDN) levels were measured by using enzyme-linked immunosorbent assay as a marker of nasal eosinophilia in all 3 groups. Results: Levels of EDN were elevated significantly in patients with PAR+RS compared with controls. Lysozyme levels were decreased significantly in patients with PAR+RS compared with PAR alone or controls. Mean lysozyme levels were significantly lower in patients with EDN levels greater than 1,000 ng/mL vs those with levels of 1,000 ng/mL or less in the PAR+RS group. There were no statistically significant differences in lactoferrin, HBD-2, and HNP-1 to 3 levels among the 3 groups. Conclusions: The presence of eosinophils and their products and reduced lysozyme concentrations may be critical factors that predispose the airways of patients with PAR to RS.
Annals of Allergy Asthma & Immunology
"Lysozyme levels in the nasal secretions of patients with perennial allergic rhinitis and recurrent sinusitis" (2004). Faculty Bibliography 2000s. 4467.