Title

Prostasin attenuates inducible nitric oxide synthase expression in lipopolysaccharide-induced urinary bladder inflammation

Authors

Authors

L. M. Chen; C. Wang; M. Q. Chen; M. R. Marcello; J. Chao; L. Chao;K. X. Chai

Comments

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Abbreviated Journal Title

Am. J. Physiol.-Renal Physiol.

Keywords

glycosylphosphatidylinositol-anchored serine protease; cytokines; cyclooxygenase-2; ESCHERICHIA-COLI LIPOPOLYSACCHARIDE; TIGHT JUNCTION DYSFUNCTION; INCREASED INOS ACTIVITY; EPITHELIAL SODIUM-CHANNEL; HUMAN SERINE; PROTEINASE; SMOOTH-MUSCLE-CELLS; ENDOTOXEMIC MICE; OUTLET OBSTRUCTION; MOLECULAR-CLONING; TRACT-INFECTIONS; Physiology; Urology & Nephrology

Abstract

Prostasin is a glycosylphosphatidylinositol- anchored serine protease, with epithelial sodium channel activation and tumor invasion suppression activities. We identified the bladder as an expression site of prostasin. In the mouse, prostasin mRNA expression was detected by reverse transcription and real-time polymerase chain reaction in the bladder, and the prostasin protein was localized by immunohistochemistry in the urothelial cells. In mice injected intraperitoneally with bacterial lipopolysaccharide (LPS), bladder prostasin mRNA expression was downregulated, whereas the expression of inducible nitric oxide synthase ( iNOS), cyclooxygenase-2 (COX-2), interferon-gamma (IFN gamma), TNF-alpha, IL-1 alpha, and IL-6 was upregulated. Viral promoter-driven expression of the human prostasin homolog in the bladder of transgenic mice attenuated the LPS induction of iNOS but did not abolish the induction. LPS induction of COX-2, TNF-alpha, IL-1 alpha, and IL-6 expression, however, was not reduced by prostasin transgene expression. Liposome-mediated delivery of prostasin-expressing plasmid into mouse bladder produced similar attenuation effects on LPS-induced iNOS expression, while not affecting COX-2 or cytokine induction. Mice receiving plasmid expressing a catalytic mutant prostasin did not manifest the iNOS induction attenuation phenotype. We propose a proteolytic mechanism for prostasin to intercept cytokine signaling during LPS-induced bladder inflammation.

Journal Title

American Journal of Physiology-Renal Physiology

Volume

291

Issue/Number

3

Publication Date

1-1-2006

Document Type

Article

Language

English

First Page

F567

Last Page

F577

WOS Identifier

WOS:000239658900007

ISSN

1931-857X

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