Title

An investigation on the analytical potential of polymerized liposomes bound to lanthanide ions for protein analysis

Authors

Authors

M. Santos; B. C. Roy; C. Goicoechea; A. D. Campiglia;S. Mallik

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

J. Am. Chem. Soc.

Keywords

DYE-BINDING; INTERFACIAL CATALYSIS; SENSITIVE METHOD; LIPID-MEMBRANES; VESICLES; SPECTROSCOPY; ASSAY; MICROARRAYS; FRACTIONS; DEVICES; Chemistry, Multidisciplinary

Abstract

We present a promising approach to protein sensing based on Eu3+ ions incorporated into polymerized liposomes. The sensitization of Eu3+ is accomplished with 5-aminosalicylic acid, which provides energy transfer for a stable reference signal and a wide wavelength excitation range free from protein interference. The lipophilic character of polymerized liposomes provides the appropriate platform for protein interaction with the lanthanide ion. Quantitative analysis is based on the linear relationship between the luminescence signal of Eu3+ and protein concentration. Because no spectral shift of the lanthanide luminescence is observed upon protein interaction, qualitative analysis is based on the luminescence lifetime of polymerized liposomes. This parameter, which changes significantly upon protein - liposome interaction, follows a well-behaved single-exponential decay that might be useful for protein identification.

Journal Title

Journal of the American Chemical Society

Volume

126

Issue/Number

34

Publication Date

1-1-2004

Document Type

Article

Language

English

First Page

10738

Last Page

10745

WOS Identifier

WOS:000223559100055

ISSN

0002-7863

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