Abbreviated Journal Title
J. Bacteriol.
Keywords
FORMATE HYDROGENLYASE SYSTEM; MOLYBDATE TRANSPORT OPERON; TRANSCRIPTIONAL ACTIVATOR; MUTATIONAL ANALYSIS; GENE-PRODUCT; NUCLEOTIDE-SEQUENCE; NIFE HYDROGENASE-1; MODE-MOLYBDATE; H-2 METABOLISM; NAR OPERONS; Microbiology
Abstract
On the basis of hyf-lacZ fusion studies, the hyf operon of Escherichia coli, noted for encoding the fourth hydrogenase isoenzyme (HYD4), is not expressed at a significant level in a wild-type strain. However, mutant FhlA proteins (constitutive activators of the hyc-encoded hydrogenase 3 isoenzyme) activated hyf-lacZ. HYfR, an FhlA homolog encoded by the hyfR gene present at the end of the hyf operon, also activated transcription of hyf-lacZ but did so only when hyfR was expressed from a heterologous promoter. The HYD4 isoenzyme did not substitute for HYD3 in H-2 production. Optimum expression of hyf-lacZ required the presence of cyclic AMP receptor protein-cyclic AMP complex and anaerobic conditions when HyfR was the activator.
Journal Title
Journal of Bacteriology
Volume
186
Issue/Number
2
Publication Date
1-1-2004
Document Type
Article
Language
English
First Page
580
Last Page
587
WOS Identifier
ISSN
0021-9193
Recommended Citation
Self, William T.; Hasona, Adnan; and Shanmugan, K. T., "Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli" (2004). Faculty Bibliography 2000s. 4787.
https://stars.library.ucf.edu/facultybib2000/4787
Comments
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