Title

Chloroplast-derived vaccine antigens and other therapeutic proteins

Authors

Authors

H. Daniell; S. Chebolu; S. Kumar; M. Singleton;R. Falconer

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Vaccine

Keywords

chloroplast genetic engineering; plant made biopharmaceuticals; oral; delivery; TRANSGENIC CHLOROPLASTS; EXPRESSION; TOBACCO; GENE; TOLERANCE; PEPTIDE; Immunology; Medicine, Research & Experimental

Abstract

The chloroplast genetic engineering offers a number of unique advantages including high level of transgene expression, multi-gene expression in single transformation event and transgene containment due to maternal inheritance. Hyper-expression of vaccine antigens or therapeutic proteins in transgenic chloroplasts (leaves) or chromoplasts (fruits/roots) facilitates efficient oral delivery. Ability of chloroplasts to correctly fold human blood proteins with proper disulfide bridges (human serum albumin or interferons) or presence of chaperones in chloroplasts to facilitate assembly of complex multi-subunit proteins or their prokaryotic nature to express native bacterial genes (up to 46.1% total leaf protein) are attractive features for therapeutic protein production. Purification of therapeutic proteins has been achieved using novel purification strategies that do not require expensive column chromatography. (c) 2004 Elsevier Ltd. All rights reserved.

Journal Title

Vaccine

Volume

23

Issue/Number

15

Publication Date

1-1-2005

Document Type

Article; Proceedings Paper

Language

English

First Page

1779

Last Page

1783

WOS Identifier

WOS:000227769600005

ISSN

0264-410X

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