Breakthrough in chloroplast genetic engineering of agronomically important crops
Abbreviated Journal Title
DAUCUS-CAROTA L.; TOBACCO CHLOROPLASTS; PLASTID TRANSFORMATION; SALT; TOLERANCE; TRANSGENIC CHLOROPLASTS; TRANSPLASTOMIC TOBACCO; STABLE; TRANSFORMATION; MATERNAL INHERITANCE; SELECTABLE MARKER; POLLEN; DISPERSAL; Biotechnology & Applied Microbiology
Chloroplast genetic engineering offers several unique advantages, including high-level transgene expression, multi-gene engineering in a single transformation event and transgene containment by maternal inheritance, as well as a lack of gene silencing, position and pleiotropic effects and undesirable foreign DNA. More than 40 trainsgenes have been stably integrated and expressed using the tobacco chloroplast genome to confer desired agronomic traits or express high levels of vaccine antigens and biopharmaceuticals. Despite such significant progress, this technology has not been extended to major crops. However, highly efficient soybean, carrot and cotton plastid transformation has recently been accomplished through somatic embryogenesis using species-specific chloroplast vectors. This review focuses on recent exciting developments in this field and offers directions for further research and development.
Trends in Biotechnology
"Breakthrough in chloroplast genetic engineering of agronomically important crops" (2005). Faculty Bibliography 2000s. 5108.