Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium
Abbreviated Journal Title
In Vitro Cell. Dev. Biol.-Anim.
adult rat spinal cord; cell culture; motoneuron; silane surface; serum-free medium; electrophysiology; FIBROBLAST-GROWTH-FACTOR; CENTRAL-NERVOUS-SYSTEM; LONG-TERM SURVIVAL; IN-VITRO; HIPPOCAMPAL-NEURONS; FACIAL-NERVE; NEUROTROPHIC FACTORS; DEFINED SYSTEM; RETINOIC ACID; MOTOR-NEURONS; Cell Biology; Developmental Biology
In this study, we have documented by morphological analysis, immunocytochemistry, and electrophysiology thedeveloptuent of a culture system that promotes the growth and long-term survival of dissociated adult rat spinal cord neurons. This system comprises a patternable, nonbiological, cell growth-promoting organosilane substrate coated oil a glass surface and all empirically derived novel serum-free medium, supplemented with specific growth factors (acidic fibroblast growth factor, heparin sulfate, neurotrophin-3. brain-derived neurotrophic factor, glial-derived neurotrophic factor, cardiotrophin-1, and vitronectin). Neurons were characterized by immunoreactivity for neurofilament 150, neuron-specific enolase, Islet-1 antibodies, electrophysiology, and the Cultures were maintained for 4-6 wk. This culture system could be a useful tool for the study of adult mammalian spinal neurons in a functional ill vitro system.
In Vitro Cellular & Developmental Biology-Animal
"Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium" (2005). Faculty Bibliography 2000s. 5109.