Title

Interference of EGFP RNA in human NT-2/D1 cell lines using human u6 promoter-based siRNA PCR products

Authors

Authors

Y. D. Kwak;K. Sugaya

Comments

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Abbreviated Journal Title

Biotechnol. Bioprocess Eng.

Keywords

gene silencing; small interference RNA; PCR; human teratocarcinoma; NT-2/D1 cells; DOUBLE-STRANDED-RNA; MAMMALIAN-CELLS; GENE-EXPRESSION; SUPPRESSION; PROTEINS; VECTOR; Biotechnology & Applied Microbiology

Abstract

RNA interference (RNAi), a process of sequence-specific gene suppression, has been known as a natural gene regulatory mechanism in a wide range of lower organisms. Recently, we have reported that a transfection of human U6 promoter (hU6) driven hairpin small-interference RNA (siRNA) plasmid specifically knocks down the target gene by posttranscriptional gene silencing in mammalian cells. Here we report that transfection of polymerase chain reaction (PCR) products, containing human U6 promoter with hairpin siRNA, knocks down the target gene expression in human teratocarcinoma NT-2/D1 cells. Moreover, we showed 3' end termination sequence, 5 Ts, is not critical elements for knocking down in PCR-based siRNA system. Therefore, the PCR-based siRNA system is a promising tool not only for the screening but also to temporally regulate gene expression in the human progenitor cells.

Journal Title

Biotechnology and Bioprocess Engineering

Volume

11

Issue/Number

3

Publication Date

1-1-2006

Document Type

Article

Language

English

First Page

273

Last Page

276

WOS Identifier

WOS:000238768600016

ISSN

1226-8372

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