Title

A water-soluble diaminostilbene derivative as a two-photon fluorescent probe

Authors

Authors

S. Yao; K. J. Schafer-Hales; I. Cohanoschi; F. E. Hernandez;K. D. Belfield

Comments

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Abbreviated Journal Title

Synlett

Keywords

two-photon; fluorescent probe; PAMAM : hydrophilic; Z-scan; two-photon; microscopy; BIS(STYRYL)BENZENE DERIVATIVES; DIPYRRYLMETHENE-BF2 DYES; COOPERATIVE; ENHANCEMENT; ABSORBING CHROMOPHORES; SUBSTITUTED STILBENES; ABSORPTION; MICROSCOPY; DONOR; DENDRIMERS; FEMTOSECOND; Chemistry, Organic

Abstract

A hydrophilic trans-4,4'-diaminostilbene derivative was prepared by the introduction of amideamino peripheral substituents. Very high aqueous solubility was achieved (> 15 mM), making it possible as a fluorescent probe for biological imaging. The relatively straightforward synthetic methodology has great potential to transform even large hydrophobic two-photon absorbing chromophores into hydrophilic derivatives. The fluorescence quantum yield of 0.27 for the diammostilbene in water is sufficiently high for use as a fluorescent probe. In addition, two-photon fluorescence images of NT2 cells stained by this probe were also obtained.

Journal Title

Synlett

Issue/Number

12

Publication Date

1-1-2006

Document Type

Article

Language

English

First Page

1863

Last Page

1866

WOS Identifier

WOS:000240009100011

ISSN

0936-5214

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