A water-soluble diaminostilbene derivative as a two-photon fluorescent probe
Abbreviated Journal Title
two-photon; fluorescent probe; PAMAM : hydrophilic; Z-scan; two-photon; microscopy; BIS(STYRYL)BENZENE DERIVATIVES; DIPYRRYLMETHENE-BF2 DYES; COOPERATIVE; ENHANCEMENT; ABSORBING CHROMOPHORES; SUBSTITUTED STILBENES; ABSORPTION; MICROSCOPY; DONOR; DENDRIMERS; FEMTOSECOND; Chemistry, Organic
A hydrophilic trans-4,4'-diaminostilbene derivative was prepared by the introduction of amideamino peripheral substituents. Very high aqueous solubility was achieved (> 15 mM), making it possible as a fluorescent probe for biological imaging. The relatively straightforward synthetic methodology has great potential to transform even large hydrophobic two-photon absorbing chromophores into hydrophilic derivatives. The fluorescence quantum yield of 0.27 for the diammostilbene in water is sufficiently high for use as a fluorescent probe. In addition, two-photon fluorescence images of NT2 cells stained by this probe were also obtained.
"A water-soluble diaminostilbene derivative as a two-photon fluorescent probe" (2006). Faculty Bibliography 2000s. 6737.