Title

Prostasin induces protease-dependent and independent molecular changes in the human prostate carcinoma cell line PC-3

Authors

Authors

M. Q. Chen; Y. Y. Fu; C. Y. Lin; L. M. Chen;K. X. Chai

Comments

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Abbreviated Journal Title

Biochim. Biophys. Acta-Mol. Cell Res.

Keywords

serine protease; matriptase; epidermal growth factor receptor; prostate; cancer; EPIDERMAL-GROWTH-FACTOR; SERINE-PROTEASE; CANCER CELLS; PLASMINOGEN-ACTIVATOR; EPITHELIAL-CELLS; TYROSINE KINASE; EXPRESSION; RECEPTOR; EGF; INHIBITORS; Biochemistry & Molecular Biology; Cell Biology

Abstract

Expression of prostasin in the PC-3 human prostate carcinoma cells inhibited in vitro invasion, but the molecular mechanisms are unknown. Wild-type human prostasin or a serine active-site mutant prostasin was expressed in the PC-3 cells. Molecular changes were measured at the mRNA and the protein levels. Cell signaling changes were evaluated by measuring phosphorylation of the extracellular signal-regulated kinases (Erk 1/2) following epidermal growth factor (EGF) treatment of the cells. Protein expression of the EGF receptor (EGFR) was differentially downregulated by the wild-type and the active-site mutant prostasin. The mRNA expression of EGFR and the transcription repressor SLUG was reduced in cells expressing wild-type prostasin but not the active-site mutant. Phosphorylation of Erk1/2 in response to EGF was greatly reduced by the wild-type prostasin but not by the active-site mutant. The mRNA expression of the urokinase-type plasminogen activator (uPA), the uPA receptor (uPAR), cyclooxygenase-2 (COX-2), and the inducible nitric oxide synthase (iNOS) was decreased by the wild-type and the active-site mutant prostasin. The mRNA or protein expression of granulocyte-macrophage colony-stimulating factor (GM-CSF), matriptase, and E-cadherin was greatly increased by the active-site mutant prostasin. In conclusion, prostasin expression elicits both protease-dependent and independent molecular changes in the PC-3 cells. (C) 2007 Elsevier B.V. All rights reserved.

Journal Title

Biochimica Et Biophysica Acta-Molecular Cell Research

Volume

1773

Issue/Number

7

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

1133

Last Page

1140

WOS Identifier

WOS:000247858600012

ISSN

0167-4889

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