mRNA profiling for body fluid identification by multiplex quantitative RT-PCR
Abbreviated Journal Title
J. Forensic Sci.
forensic science; RNA; mRNA profiling; body fluid identification; blood; identification; saliva identification; semen identification; menstrual; blood identification; multiplex real-time PCR; DELTA-AMINOLEVULINATE SYNTHASE; GENE; Medicine, Legal
An alternative approach to conventional protein-based body fluid identification is gene expression profiling analysis. In the present work, we report the development of sensitive and robust multiplex quantitative reverse transcriptase-PCR assays for the identification of blood, saliva, semen, and menstrual blood. Each body fluid assay comprises a triplex system that detects transcripts from two body fluid-specific genes and a housekeeping gene GAPDH. The body fluid-specific genes include erythroid delta-aminolevulinate synthase (ALAS2) and beta-spectrin (SPTB) for blood, statherin (STATH) and histatin 3 (HTN3) for saliva, protamine 1 (PRM1) and protamine 2 (PRM2) for semen, and matrix metalloproteinase 7 (MMP7) and matrix metalloproteinase 10 (MMP10) for menstrual blood. Normalization of both body fluid-specific genes to the housekeeping gene by means of appropriate cycle threshold metrics ensures the high specificity of each assay for its cognate body fluid.
Journal of Forensic Sciences
Article; Proceedings Paper
"mRNA profiling for body fluid identification by multiplex quantitative RT-PCR" (2007). Faculty Bibliography 2000s. 7275.