Title

mRNA profiling for body fluid identification by multiplex quantitative RT-PCR

Authors

Authors

J. Juusola;J. Ballantyne

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

J. Forensic Sci.

Keywords

forensic science; RNA; mRNA profiling; body fluid identification; blood; identification; saliva identification; semen identification; menstrual; blood identification; multiplex real-time PCR; DELTA-AMINOLEVULINATE SYNTHASE; GENE; Medicine, Legal

Abstract

An alternative approach to conventional protein-based body fluid identification is gene expression profiling analysis. In the present work, we report the development of sensitive and robust multiplex quantitative reverse transcriptase-PCR assays for the identification of blood, saliva, semen, and menstrual blood. Each body fluid assay comprises a triplex system that detects transcripts from two body fluid-specific genes and a housekeeping gene GAPDH. The body fluid-specific genes include erythroid delta-aminolevulinate synthase (ALAS2) and beta-spectrin (SPTB) for blood, statherin (STATH) and histatin 3 (HTN3) for saliva, protamine 1 (PRM1) and protamine 2 (PRM2) for semen, and matrix metalloproteinase 7 (MMP7) and matrix metalloproteinase 10 (MMP10) for menstrual blood. Normalization of both body fluid-specific genes to the housekeeping gene by means of appropriate cycle threshold metrics ensures the high specificity of each assay for its cognate body fluid.

Journal Title

Journal of Forensic Sciences

Volume

52

Issue/Number

6

Publication Date

1-1-2007

Document Type

Article; Proceedings Paper

Language

English

First Page

1252

Last Page

1262

WOS Identifier

WOS:000251553700003

ISSN

0022-1198

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