Title

Photolithographic patterning of C2C12 myotubes using vitronectin as growth substrate in serum-free medium

Authors

Authors

P. Molnar; W. S. Wang; A. Natarajan; J. W. Rumsey;J. J. Hickman

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Biotechnol. Prog.

Keywords

SMOOTH-MUSCLE-CELLS; SKELETAL-MUSCLE; IN-VITRO; HIPPOCAMPAL-NEURONS; PRINTING METHOD; TISSUE; DIFFERENTIATION; MYOBLASTS; MOUSE; EXPRESSION; Biotechnology & Applied Microbiology; Food Science & Technology

Abstract

The C2C12 cell line is frequently used as a model of skeletal muscle differentiation. In our serum-free defined culture system, differentiation of C2C12 cells into myotubes required surface-bound signals such as substrate-adsorbed vitronectin or laminin. On the basis of this substrate requirement of myotube formation, we developed a photolithography-based method to pattern C2C12 myotubes, where myotubes formed exclusively on vitronectin surface patterns. We have determined that the optimal line width to form single myotubes is approximately 30 mu m. To illustrate a possible application of this method, we patterned myotubes on the top of commercial substrate-embedded microelectrodes. In contrast to previous experiments where cell patterning was achieved by selective attachment of the cells to patterned surfaces in a medium that contained all of the factors necessary for differentiation, this study illustrates that surface patterning of a signaling molecule, which is essential for skeletal muscle differentiation in a defined system, can result in the formation of aligned myotubes on the patterns. This technique is being developed for applications in cell biology, tissue engineering, and robotics.

Journal Title

Biotechnology Progress

Volume

23

Issue/Number

1

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

265

Last Page

268

WOS Identifier

WOS:000243927600036

ISSN

8756-7938

Share

COinS