Distribution of DNA in cationic liposome complexes probed by Raman microscopy
Abbreviated Journal Title
ATOMIC-FORCE MICROSCOPY; GENE-TRANSFER; CONDENSATION; PHASE; EXPRESSION; TRANSITION; DYNAMICS; DELIVERY; THERAPY; HUMANS; Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, ; Multidisciplinary
The potential application of Raman microscopy to image the distribution of DNA inside lipid-DNA complexes was examined. To test this, we mixed DNA with DOTMA/DOPE liposome vesicles and imaged the distribution of DNA in the liposome complex by using the characteristic DNA guanine peak as a marker. The Raman intensity map indicates that the concentration of DNA molecules is higher near the core of the DOTMA/DOPE liposome vesicle. This result demonstrates that this technique is a viable technique to monitor DNA distribution inside liposomes.
"Distribution of DNA in cationic liposome complexes probed by Raman microscopy" (2001). Faculty Bibliography 2000s. 8122.