Title

Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens

Authors

Authors

N. A. Riza; M. Sheikh; G. Webb-Wood;P. G. Kik

Comments

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Abbreviated Journal Title

Opt. Eng.

Keywords

liquid crystal; optical imaging; microscopy; BRAGG GRATINGS; SCANNER; ABERRATION; SYSTEMS; MIRROR; Optics

Abstract

Three-dimensional (3-D) imaging is demonstrated using an electronically controlled liquid crystal (LC) optical lens to accomplish a no-moving-parts depth-section scanning in a modified commercial 3-D confocal microscope. Specifically, 3-D views of a standard CDC blood vessel (enclosed in a glass slide) have been obtained using the modified confocal microscope operating at the red 633-nm laser wavelength. The image sizes over a 25-mu m axial scan depth were 50 x 50 mu m and 80 x 80 mu m, using 60x and 20x micro-objectives, respectively. The transverse motion step was 0.1 mu m for the 60x data and 0.2 mu m for the 20x data. As a first-step comparison, image processing of the standard and LC electronic-lens microscope images indicates correlation values between 0.81 and 0.91. The proposed microscopy system within aberration limits has the potential to eliminate the mechanical forces due to sample or objective motion that can distort the original sample structure and lead to imaging errors. (C) 2008 Society of Photo-Optical Instrumentation Engineers.

Journal Title

Optical Engineering

Volume

47

Issue/Number

6

Publication Date

1-1-2008

Document Type

Article

Language

English

First Page

9

WOS Identifier

WOS:000257884800005

ISSN

0091-3286

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