Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe
Abbreviated Journal Title
Fluorescent dyes; Pluronic; Multiphoton absorption; Bioimaging; Fluorescence microscopy; Lysosomes; STIMULATED-EMISSION-DEPLETION; OPTICAL-DATA STORAGE; FLUORENE; DERIVATIVES; CROSS-SECTIONS; BECLIN 1; MICROSCOPY; AUTOPHAGY; RESOLUTION; TRANSPORT; NM; Biochemical Research Methods; Chemistry, Analytical; Chemistry, Physical
We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic (R) F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.
Journal of Fluorescence
"Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe" (2011). Faculty Bibliography 2010s. 1063.