Title

mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise

Authors

Authors

C. Haas; E. Hanson; W. Bar; R. Banemann; A. M. Bento; A. Berti; E. Borges; C. Bouakaze; A. Carracedo; M. Carvalho; A. Choma; M. Dotsch; M. Duriancikova; P. Hoff-Olsen; C. Hohoff; P. Johansen; P. A. Lindenbergh; B. Loddenkotter; B. Ludes; O. Maronas; N. Morling; H. Niederstatter; W. Parson; G. Patel; C. Popielarz; E. Salata; P. M. Schneider; T. Sijen; B. Sviezena; L. Zatkalikova;J. Ballantyne

Comments

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Abbreviated Journal Title

Forensic Sci. Int.-Genet.

Keywords

Forensic science; mRNA profiling; Body fluid identification; Blood; EDNAP exercise; BODY-FLUID IDENTIFICATION; RT-PCR; MENSTRUAL BLOOD; SALIVA STAINS; MARKERS; SEMEN; Genetics & Heredity; Medicine, Legal

Abstract

A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

Journal Title

Forensic Science International-Genetics

Volume

5

Issue/Number

1

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

21

Last Page

26

WOS Identifier

WOS:000286134200018

ISSN

1872-4973

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