Title

Cellular Infiltration and Cytokine Expression Correlate with Fistulizing State in Crohn's Disease

Authors

Authors

S. A. Naser; C. Romero; P. Urbina; N. Naser;J. Valentine

Comments

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Abbreviated Journal Title

Clin. Vaccine Immunol.

Keywords

INFLAMMATORY-BOWEL-DISEASE; ULCERATIVE-COLITIS; RESPONSES; RECEPTOR; PROTEIN; CELLS; Immunology; Infectious Diseases; Microbiology

Abstract

The purpose of this study was to determine the degree of infiltration of different cell subpopulations (tissue dendritic macrophages, T-helper cells, cytotoxic T lymphocytes, monocytes, neutrophils, and B cells) and the expression of the cytokines interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-alpha) in inflamed and noninflamed resected tissues from Crohn's disease (CD) and non-CD patients. Twenty-one resected full-thickness intestinal tissue specimens representing 13 subjects (8 CD and 5 non-CD patients) were included in this study. Sections of 20 mu m in thickness were cut and then stained using immunohistochemistry. The sections were analyzed using confocal laser scanning microscopy (CLSM). Patterns of staining for inflamed CD and noninflamed CD tissues versus non-CD tissues demonstrated significant differences in the macrophage and T-helper subpopulations. Surprisingly, the T-helper subset was decreased significantly in the inflamed CD sections compared to the noninflamed CD and non-CD sections. The staining patterns also suggested differences in the expression of both IL-12 and TNF-alpha between the groups, with cytokine overexpression directly relating to the fistulizing state in CD patients. Cytokine expression is upregulated in chronic CD patients; therefore, the degree of inflammation and tissue damage in CD is dependent on the expression of specific cytokines within the tissue. Differentiation of cell subpopulations may be important for establishing a direct relationship with each state of CD (inflammatory, stricturing, and fistulizing states).

Journal Title

Clinical and Vaccine Immunology

Volume

18

Issue/Number

9

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

1416

Last Page

1419

WOS Identifier

WOS:000294452800004

ISSN

1556-6811

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