Title

Multiplexed biomarker detection using x-ray fluorescence of composition-encoded nanoparticles

Authors

Authors

M. Hossain; C. M. Wang;M. Su

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Appl. Phys. Lett.

Keywords

PROSTATE-CANCER; BREAST; TISSUES; ELEMENTS; COLON; BONE; LEAD; Physics, Applied

Abstract

Multiple DNA and protein biomarkers have been detected based on characteristic x-ray fluorescence of a panel of metal and alloy nanoparticles, which are modified with ligands of biomarkers to create a one-to-one correspondence and immobilized on ligand-modified substrates after forming complexes with target biomarkers in three-strand or sandwich configuration. By determining the presence and concentration of nanoparticles using x-ray fluorescence, the nature and amount of biomarkers can be detected with limits of 1 nM for DNA and 1 ng/ml for protein. By combining high penetrating ability of x-rays, this method allows quantitative imaging of multiple biomarkers. (C) 2010 American Institute of Physics. [doi:10.1063/1.3533817]

Journal Title

Applied Physics Letters

Volume

97

Issue/Number

26

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

3

WOS Identifier

WOS:000285768100093

ISSN

0003-6951

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