Multiplexed biomarker detection using x-ray fluorescence of composition-encoded nanoparticles
Abbreviated Journal Title
Appl. Phys. Lett.
PROSTATE-CANCER; BREAST; TISSUES; ELEMENTS; COLON; BONE; LEAD; Physics, Applied
Multiple DNA and protein biomarkers have been detected based on characteristic x-ray fluorescence of a panel of metal and alloy nanoparticles, which are modified with ligands of biomarkers to create a one-to-one correspondence and immobilized on ligand-modified substrates after forming complexes with target biomarkers in three-strand or sandwich configuration. By determining the presence and concentration of nanoparticles using x-ray fluorescence, the nature and amount of biomarkers can be detected with limits of 1 nM for DNA and 1 ng/ml for protein. By combining high penetrating ability of x-rays, this method allows quantitative imaging of multiple biomarkers. (C) 2010 American Institute of Physics. [doi:10.1063/1.3533817]
Applied Physics Letters
"Multiplexed biomarker detection using x-ray fluorescence of composition-encoded nanoparticles" (2010). Faculty Bibliography 2010s. 267.