Title

Purification, crystallization and X-ray diffraction analysis of human dynamin-related protein 1 GTPase-GED fusion protein

Authors

Authors

E. Klinglmayr; J. Wenger; S. Mayr; E. Bossy-Wetzel;S. Puehringer

Comments

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Abbreviated Journal Title

Acta Crystallogr. F-Struct. Biol. Cryst. Commun.

Keywords

MITOCHONDRIAL FISSION; ALZHEIMERS-DISEASE; ENZYMATIC-ACTIVITY; MAMMALIAN-CELLS; S-NITROSYLATION; DRP1; DOMAIN; NEURODEGENERATION; MECHANISM; APOPTOSIS; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography

Abstract

The mechano-enzyme dynamin-related protein 1 plays an important role in mitochondrial fission and is implicated in cell physiology. Dysregulation of Drp1 is associated with abnormal mitochondrial dynamics and neuronal damage. Drp1 shares structural and functional similarities with dynamin 1 with respect to domain organization, ability to self-assemble into spiral-like oligomers and GTP-cycle-dependent membrane scission. Structural studies of human dynamin-1 have greatly improved the understanding of this prototypical member of the dynamin superfamily. However, high-resolution structural information for full-length human Drp1 covering the GTPase domain, the middle domain and the GTPase effector domain (GED) is still lacking. In order to obtain mechanistic insights into the catalytic activity, a nucleotide-free GTPase-GED fusion protein of human Drp1 was expressed, purified and crystallized. Initial X-ray diffraction experiments yielded data to 2.67 angstrom resolution. The hexagonal-shaped crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 53.59, b = 151.65, c = 43.53 angstrom, one molecule per asymmetric unit and a solvent content of 42%. Expression of selenomethionine-labelled protein is currently in progress. Here, the expression, purification, crystallization and X-ray diffraction analysis of the Drp1 GTPase-GED fusion protein are presented, which form a basis for more detailed structural and biophysical analysis.

Journal Title

Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Volume

68

Publication Date

1-1-2012

Document Type

Article

Language

English

First Page

1217

Last Page

1221

WOS Identifier

WOS:000309357200016

ISSN

1744-3091

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