Title

Proline-Dependent Regulation of Clostridium difficile Stickland Metabolism

Authors

Authors

L. Bouillaut; W. T. Self;A. L. Sonenshein

Comments

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Abbreviated Journal Title

J. Bacteriol.

Keywords

ANAEROBES GENUS CLOSTRIDIUM; TOXIN PRODUCTION; GLYCINE REDUCTASE; BACILLUS-SUBTILIS; CL.-SPOROGENES; SIGMA-FACTOR; AMINO-ACIDS; SELENOPROTEIN COMPONENT; ENZYMIC REDUCTION; STATIONARY-PHASE; Microbiology

Abstract

Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, generating ATP and reducing power in the process. Reduction of the electron acceptors proline and glycine requires the D-proline reductase (PR) and the glycine reductase (GR) enzyme complexes, respectively. Addition of proline in the medium increases the level of PR protein but decreases the level of GR. We report the identification of PrdR, a protein that activates transcription of the PR-encoding genes in the presence of proline and negatively regulates the GR-encoding genes. The results suggest that PrdR is a central metabolism regulator that controls preferential utilization of proline and glycine to produce energy via the Stickland reactions.

Journal Title

Journal of Bacteriology

Volume

195

Issue/Number

4

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

844

Last Page

854

WOS Identifier

WOS:000316961200022

ISSN

0021-9193

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