Title

Chlamydia trachomatis Tarp Harbors Distinct G and F Actin Binding Domains That Bundle Actin Filaments

Authors

Authors

S. Jiwani; S. Alvarado; R. J. Ohr; A. Romero; B. Nguyen;T. J. Jewett

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

J. Bacteriol.

Keywords

PROTEIN; CYTOSKELETON; RECRUITMENT; INVASION; PHOSPHORYLATION; POLYMERIZATION; SALMONELLA; PROMOTE; COMPLEX; FAMILY; Microbiology

Abstract

All species of Chlamydia undergo a unique developmental cycle that transitions between extracellular and intracellular environments and requires the capacity to invade new cells for dissemination. A chlamydial protein called Tarp has been shown to nucleate actin in vitro and is implicated in bacterial entry into human cells. Colocalization studies of ectopically expressed enhanced green fluorescent protein (EGFP)-Tarp indicate that actin filament recruitment is restricted to the C-terminal half of the effector protein. Actin filaments are presumably associated with Tarp via an actin binding alpha helix that is also required for actin nucleation in vitro, but this has not been investigated. Tarp orthologs from C. pneumoniae, C. muridarum, and C. caviae harbor between 1 and 4 actin binding domains located in the C-terminal half of the protein, but C. trachomatis serovar L2 has only one characterized domain. In this work, we examined the effects of domain-specific mutations on actin filament colocalization with EGFP-Tarp. We now demonstrate that actin filament colocalization with Tarp is dependent on two novel F-actin binding domains that endow the Tarp effector with actin-bundling activity. Furthermore, Tarp-mediated actin bundling did not require actin nucleation, as the ability to bundle actin filaments was observed in mutant Tarp proteins deficient in actin nucleation. These data shed molecular insight on the complex cytoskeletal rearrangements required for C. trachomatis entry into host cells.

Journal Title

Journal of Bacteriology

Volume

195

Issue/Number

4

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

708

Last Page

716

WOS Identifier

WOS:000316961200008

ISSN

0021-9193

Share

COinS