Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection of MicroRNA
Abbreviated Journal Title
Biochim. Biophys. Acta-Gen. Subj.
CIRCULATING MICRORNAS; PLASMA; PROBE; BIOSENSORS; SEQUENCES; IMPEDANCE; SENSORS; MARKERS; Chemistry, Analytical
MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (similar to 36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.
"Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection of MicroRNA" (2013). Faculty Bibliography 2010s. 4254.