Title

Amperometric carbon fiber nitrite microsensor for in situ biofilm monitoring

Authors

Authors

W. H. Lee; D. G. Wahman;J. G. Pressman

Comments

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Abbreviated Journal Title

Public Perform. Manag. Rev.

Keywords

Biofilm; Carbon fiber; In situ monitoring; Microsensor; Nitrification; Nitrite; ELECTROCHEMICAL DETERMINATION; NITRIFYING BIOFILMS; ELECTRODE; MICROELECTRODE; OXIDATION; NITRATE; SURFACE; ION; VOLTAMMETRY; REDUCTION; Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation

Abstract

A highly selective needle type solid state amperometric nitrite microsensor based on direct nitrite oxidation on carbon fiber was developed using a simplified fabrication method. The microsensor's tip diameter was approximately 7 mu m (14 mu m spatial resolution). At an applied potential of +1.2 V vs. Ag/AgCl, the microsensor exhibited a linear nitrite response from 0 to 25 mg N L-1, a 0.02 mg N L-1(1.3 mu M) limit of detection, and a fast response (<5 s). There was minimal interference with less than 3% of electrode response changes from major chemicals of interest in drinking water and wastewater systems [oxygen, ammonium, monochloramine, nitrate, sodium bicarbonate (alkalinity), chloride, sulfate, and acetate]. Hydrogen ion (pH) affected nitrite measurement by shifting the baseline response, translating into an approximate change of 0.24 mg N L-1 nitrite per 1 pH unit change. Depending on the conditions (e. g., pH, alkalinity, nitrite concentration), pH may need to be taken into account during nitrite measurement. The developed carbon fiber nitrite microsensor successfully measured nitrite in a nitrifying biofilm and is applicable for in situ analysis in other micro-environments (e. g., microbial mats and sediments). Published by Elsevier B.V.

Journal Title

Sensors and Actuators B-Chemical

Volume

Sens. Actuator B-Chem.

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

1263

Last Page

1269

WOS Identifier

188

ISSN

0925-4005

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