Title

Novel technique for generating macrophage foam cells for in vitro reverse cholesterol transport studies

Authors

Authors

B. Sengupta; C. A. Narasimhulu;S. Parthasarathy

Comments

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Abbreviated Journal Title

J. Lipid Res.

Keywords

lysophosphatidylcholine; high density lipoprotein; NBD-cholesterol; HIGH-DENSITY-LIPOPROTEIN; SCAVENGER RECEPTOR; ALPHA-TOCOPHEROL; EFFLUX; CAPACITY; HUMAN-MONOCYTES; ATHEROSCLEROSIS; METABOLISM; LYSOLECITHIN; HDL; LYSOPHOSPHATIDYLCHOLINE; Biochemistry & Molecular Biology

Abstract

Generation of foam cells, an essential step for reverse cholesterol transport studies, uses the technique of receptor-dependent macrophage loading with radiolabeled acetylated LDL. In this study, we used the ability of a biologically relevant detergent molecule, lysophosphatidylcholine (lyso-PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabeled cholesterol/lyso-PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4 degrees C and retained the solubilized cholesterol during one month of storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently labeled, or radiolabeled)/lyso-PtdCho mixed micelles accumulated CE as documented by microscopy, lipid staining, labeled oleate incorporation, and by TLC. Such foam cells unloaded cholesterol when incubated with HDL but not with oxidized HDL. We propose that stable cholesterol or CE/lyso-PtdCho micelles would offer advantages over existing methods.

Journal Title

Journal of Lipid Research

Volume

54

Issue/Number

12

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

3358

Last Page

3372

WOS Identifier

WOS:000330534900013

ISSN

0022-2275

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