Title

Deep Vascular Imaging in Wounds by Two-Photon Fluorescence Microscopy

Authors

Authors

C. O. Yanez; A. R. Morales; X. L. Yue; T. Urakami; M. Komatsu; T. A. H. Jarvinen;K. D. Belfield

Comments

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Abbreviated Journal Title

PLoS One

Keywords

CYCLIC RGD PEPTIDES; TISSUE; ALPHA(V)BETA(3); RECOGNITION; INTEGRINS; Multidisciplinary Sciences

Abstract

Deep imaging within tissue (over 300 mu m) at micrometer resolution has become possible with the advent of two-photon fluorescence microscopy (2PFM). The advantages of 2PFM have been used to interrogate endogenous and exogenous fluorophores in the skin. Herein, we employed the integrin (cell-adhesion proteins expressed by invading angiogenic blood vessels) targeting characteristics of a two-photon absorbing fluorescent probe to image new vasculature and fibroblasts up to approximate to 1600 mu m within wound (neodermis)/granulation tissue in lesions made on the skin of mice. Reconstruction revealed three dimensional (3D) architecture of the vascular plexus forming at the regenerating wound tissue and the presence of a fibroblast bed surrounding the capillaries. Biologically crucial events, such as angiogenesis for wound healing, may be illustrated and analyzed in 3D on the whole organ level, providing novel tools for biomedical applications.

Journal Title

Plos One

Volume

8

Issue/Number

7

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

6

WOS Identifier

WOS:000321341000056

ISSN

1932-6203

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