A Differential Fluorescent Receptor for Nucleic Acid Analysis
Abbreviated Journal Title
differential receptors; dumbbell molecular beacon; principal component; analysis; SNP analysis; RESISTANT MYCOBACTERIUM-TUBERCULOSIS; DOUBLE-CROSSOVER MOLECULES; TIME; PCR ASSAY; RIFAMPIN RESISTANCE; XPERT MTB/RIF; SENSOR ARRAYS; EXTRAPULMONARY TUBERCULOSIS; SPECIES IDENTIFICATION; PATTERN-RECOGNITION; REACTIVE ARRAYS; Biochemistry & Molecular Biology; Chemistry, Medicinal
Differential receptors use an array of sensors to recognize analytes. Each sensor in the array can recognize not one, but several analytes with different rates, so a single analyte triggers a response of several sensors in the array. The receptor thus produces a pattern of signals that is unique for each analyte, thereby enabling identification of a specific analyte by producing a fingerprint pattern. We applied this approach for the analysis of DNA sequences of Mycobacterium tuberculosis strains that differ by single nucleotide substitutions in the 81-bp hot-spot region that imparts rifampin resistance. The technology takes advantage of the new multicomponent, selfassembling sensor, which produces a fluorescent signal in the presence of specific DNA sequences. A differential fluorescent receptor (DFR) contained an array of three such sensors and differentiated at least eight DNA sequences. The approach requires only one molecular-beacon-like fluorescent reporter, which can be used by all three sensors. The DFR developed in this study represents a cost-efficient alternative to molecular diagnostic technologies that use fluorescent hybridization probes.
"A Differential Fluorescent Receptor for Nucleic Acid Analysis" (2014). Faculty Bibliography 2010s. 5065.