Title

A host-specific factor is necessary for efficient folding of the autotransporter plasmid-encoded toxin

Authors

Authors

K. N. Nemec; P. Scaglione; F. Navarro-Garcia; J. Huerta; S. A. Tatulian;K. Teter

Comments

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Abbreviated Journal Title

Biochimie

Keywords

Autotransporter; Circular dichroism; Plasmid-encoded toxin; Protein; folding; ENTEROAGGREGATIVE ESCHERICHIA-COLI; SHIGELLA-FLEXNERI; PROTEIN; SECRETION; EPITHELIAL-CELLS; COMMON THEMES; BETA-HELIX; PET; PATHWAY; SURFACE; ICSA; Biochemistry & Molecular Biology

Abstract

Autotransporters are the most common virulence factors secreted from Gram-negative pathogens. Until recently, autotransporter folding and outer membrane translocation were thought to be self-mediated events that did not require accessory factors. Here, we report that two variants of the autotransporter plasmid-encoded toxin are secreted by a lab strain of Escherichia coli. Biophysical analysis and cell-based toxicity assays demonstrated that only one of the two variants was in a folded, active conformation. The misfolded variant was not produced by a pathogenic strain of enteroaggregative E. coli and did not result from protein overproduction in the lab strain of E coli. Our data suggest a host-specific factor is required for efficient folding of plasmid-encoded toxin. (C) 2009 Elsevier Masson SAS. All rights reserved.

Journal Title

Biochimie

Volume

92

Issue/Number

2

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

171

Last Page

177

WOS Identifier

WOS:000274610600007

ISSN

0300-9084

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