Utilization of Microscale Silicon Cantilevers to Assess Cellular Contractile Function In Vitro
Abbreviated Journal Title
J. Vis. Exp.
Bioengineering; Issue 92; cantilever; in vitro; contraction; skeletal; muscle; NMJ; cardiomyocytes; functional; SKELETAL-MUSCLE; SINGLE-FIBER; MOTONEURONS; CULTURE; GROWTH; MODEL; EXCITABILITY; INTEGRATION; CELLS; YOUNG; Multidisciplinary Sciences
The development of more predictive and biologically relevant in vitro assays is predicated on the advancement of versatile cell culture systems which facilitate the functional assessment of the seeded cells. To that end, microscale cantilever technology offers a platform with which to measure the contractile functionality of a range of cell types, including skeletal, cardiac, and smooth muscle cells, through assessment of contraction induced substrate bending. Application of multiplexed cantilever arrays provides the means to develop moderate to high-throughput protocols for assessing drug efficacy and toxicity, disease phenotype and progression, as well as neuromuscular and other cell-cell interactions. This manuscript provides the details for fabricating reliable cantilever arrays for this purpose, and the methods required to successfully culture cells on these surfaces. Further description is provided on the steps necessary to perform functional analysis of contractile cell types maintained on such arrays using a novel laser and photo-detector system. The representative data provided highlights the precision and reproducible nature of the analysis of contractile function possible using this system, as well as the wide range of studies to which such technology can be applied. Successful widespread adoption of this system could provide investigators with the means to perform rapid, low cost functional studies in vitro, leading to more accurate predictions of tissue performance, disease development and response to novel therapeutic treatment.
Jove-Journal of Visualized Experiments
"Utilization of Microscale Silicon Cantilevers to Assess Cellular Contractile Function In Vitro" (2014). Faculty Bibliography 2010s. 6097.