Title

A multiplexed chip-based assay system for investigating the functional development of human skeletal myotubes in vitro

Authors

Authors

A. S. T. Smith; C. J. Long; K. Pirozzi; S. Najjar; C. McAleer; H. H. Vandenburgh;J. J. Hickman

Comments

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Abbreviated Journal Title

J. Biotechnol.

Keywords

Human; Skeletal muscle; Cantilevers; Functional assay; Body-on-a-chip; CONTRACTILE PROPERTIES; MUSCLE; CELLS; EXCITABILITY; INTEGRATION; CULTURE; TISSUES; 3D; Biotechnology & Applied Microbiology

Abstract

This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens. (C) 2014 Elsevier B.V. All rights reserved.

Journal Title

Journal of Biotechnology

Volume

185

Publication Date

1-1-2014

Document Type

Article

Language

English

First Page

15

Last Page

18

WOS Identifier

WOS:000341046100003

ISSN

0168-1656

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