Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general
Abbreviated Journal Title
SURFACE-PLASMON RESONANCE; HORSERADISH-PEROXIDASE; CANCER BIOMARKERS; PROSTATE-CANCER; ANTIBODY-AFFINITY; PROTEIN-DETECTION; CHEMILUMINESCENCE; SENSITIVITY; NANOTECHNOLOGY; NANOPARTICLES; Chemistry, Analytical
The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the detection limit can help researchers to evaluate different bio-analytical techniques properly, and to design better strategies to optimize and achieve the best analytical performance.
"Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general" (2014). Faculty Bibliography 2010s. 6361.