Title

Oxidized low-density lipoprotein alters endothelial progenitor cell populations

Authors

Authors

Y. Q. Cui; C. A. Narasimhulu; L. J. Liu; X. Li; Y. Xiao; J. Zhang; X. Y. Xie; H. Hao; J. Z. Liu; G. L. He; P. J. Cowan; L. Q. Cui; H. Zhu; S. Parthasarathy;Z. G. Liu

Comments

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Abbreviated Journal Title

Front. Biosci.

Keywords

ox-LDL; hyperlipidemia; NAC; ROS; EPC; CORONARY-ARTERY DISEASE; SMOOTH-MUSCLE-CELLS; MARROW STEM-CELLS; NEOINTIMA FORMATION; PERIPHERAL-BLOOD; VASCULAR BIOLOGY; OXIDATIVE; STRESS; PROTEIN-KINASE; HEART-FAILURE; PLASMA-LEVELS; Biochemistry & Molecular Biology; Cell Biology

Abstract

Oxidized low-density lipoprotein (ox-LDL) is critical to atherosclerosis in hyperlipidemia. Bone marrow (BM)-derived endothelial progenitor cells (EPCs) are important in preventing atherosclerosis, however these cells are significantly decreased in number in hyperlipidemia. This study aimed to determine whether ox-LDL and hyperlipidemia exert similar effects on EPC populations, and to investigate the involvement of reactive oxygen species (ROS). ROS production in BM and blood was significantly increased in male C57BL/6 mice treated with intravenous ox-LDL, and in hyperlipidemic LDL receptor knockout mice fed with a 4-month high-fat diet. ROS formation was effectively blocked by overexpression of antioxidant enzymes or N-acetylcysteine treatment. In both hyperlipidemic and ox-LDL-treated mice, the number of c-Kit(+)/CD31(+) cells in BM and blood and of Sca-1(+)/Flk-1(+) cells in blood significantly decreased, whereas the number of blood Flk-1(+) cells increased. In contrast, the number of blood CD34(+)/CD133(+) cells increased in ox-LDL-treated mice but decreased in hyperlipidemic mice. Only the changes in CD34(+)/Flk-1(+) cell number were prevented by inhibiting ROS production. These data suggested that ox-LDL produced significant changes in BM and blood EPC populations, largely similar to chronic hyperlipidemia, via predominantly ROS-independent mechanism(s).

Journal Title

Frontiers in Bioscience-Landmark

Volume

20

Publication Date

1-1-2015

Document Type

Article

Language

English

First Page

975

Last Page

988

WOS Identifier

WOS:000354355900007

ISSN

1093-9946

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