Mulan is an E3 ubiquitin ligase and an E3 SUMO ligase embedded in the outer mitochondrial membrane. Mulan plays a major role in various cell processes including cell growth, mitophagy, apoptosis, and mitochondrial dynamics. In addition, its deregulation isinvolved in the development and progression of several human disorders such as neurodegeneration and heart disease. There are two main discernible domains in Mulan: a large cytoplasmic domain that encodes the RING-finger motif and carries out the catalytic activity of the protein; the second domain of Mulan is exposed to the intermembrane space of mitochondria, and its function remains unknown. This part of Mulan is also referred to as the BAM domain and is expected to have a significant function since its amino acid sequence has been conserved through evolution and is found in bacteria, animals, and plants. The purpose of this study is to isolate and characterize potential binding partner proteins of the BAM domain using the yeast two-hybrid system. These studies are expected to provide new information on the physiological function of this domain and how it is potentially used to modulate the ligase activity of Mulan.
Zervos, Antonis S.
Bachelor of Science (B.S.)
College of Medicine
Burnett School of Biomedical Sciences
Orlando (Main) Campus
Length of Campus-only Access
Herbert, Jared M., "The Role of the Intermembrane Domain of Mulan in Mitophagy and Cell Death" (2016). Honors in the Major Theses. 235.