Expression of protective antigens in transgenic chloroplasts and the production of improved vaccines (EP)
Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The invention further provides transformed plastids having the ability to survive selection in both the light and the dark, at different developmental stages by using genes coding for two different enzymes capable of detoxifying the same selectable marker, driven by regulatory signals that are functional in proplastids as well as in mature chloroplasts. The invention utilizes antibiotic-free selectable markers to provide edible vaccines for conferring immunity to a mammal against Bacillus anthracis, as well as Yersina pestis. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants.
EP 1 456 390 B1
Application Serial Number
Agency: University of Pennsylvania
Assignee at Issuance
Burnett School of Biomedical Sciences
Molecular Biology & Microbiology
Assignee at Filing
National Filing Record
Daniell, Henry, "Expression of protective antigens in transgenic chloroplasts and the production of improved vaccines (EP)" (2011). UCF Patents. 171.