A method of transforming plant plastids and regenerating fertile transplastomic plants by somatic embryogenesis is disclosed. The method involves transforming a plant plastid in a plant cell capable of being regenerated through somatic embryogenesis with a plastid expression cassette comprising one or more selectable marker genes that express in both green and non-green tissue and in light and dark conditions wherein the selectable marker plant cell is cultured in the presence of the plant cell to a selection agent. The transplastomic plant cell is cultured in the presence of the selection agent under conditions to cause the formation of a somatic embryo. The somatic embryo is grown into a fertile transplastomic plant. Preferably, the expression cassette contains two different selectable marker genes that express different proteins that provide plant cell resistance to the same selection agent.
Application Serial Number
Agency: University of Pennsylvania
Assignee at Issuance
Burnett School of Biomedical Sciences
Molecular Biology & Microbiology
Assignee at Filing
Nonprovisional Application Record
Daniell, Henry; Kumar, Shashi; and Kumar, Shashi, "Plastid genetic engineering via somatic embryogenisis" (2010). UCF Patents. 461.