Electrochemical biosensors often employ enzymes as detection elements. These sensors are highly selective towards target analytes, however the scope of their application is limited by the poor stability of the enzyme. In this study, multi-valent inorganic cerium oxide nanoparticles were used as detection elements for the analysis of hydrogen peroxide. The electrochemical response of the cerium oxide towards hydrogen peroxide analyte is defined through cyclic voltammetry and chronoamperometry. This response was found to be dependent on nanoparticle Ce3+:Ce4+ redox state ratio and this property is exploited to fabricate a biosensor. As produced, the biosensor demonstrated sensitivity at picomolar analyte concentrations. Further, the sensitivity of the electrode is stable across a range of temperatures and pH's which inhibit the function of standard enzyme-based sensors. Additionally, the produced sensor retained function in sheep serum demonstrating the high selectivity and robustness of the sensor.
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Master of Science in Materials Science and Engineering (M.S.M.S.E.)
College of Engineering and Computer Science
Materials Science Engineering
Materials Science and Engineering
Length of Campus-only Access
Masters Thesis (Open Access)
Neal, Craig, "Fabrication and Investigation of an enzyme-free, Nanoparticle-based Biosensor for Hydrogen Peroxide determination" (2016). Electronic Theses and Dissertations, 2004-2019. 5141.