ORCID

0009-0000-7726-9436

Keywords

3D MEAs, Mitochondria, Biosensor, Electrophysiology, Metabolism, Electrochemical Impedance Spectroscopy

Abstract

Developing new label-free paradigms for functional assays in biomedical research has the potential to catalyze efforts in drug discovery and improve the understanding of complex disorders. Mitochondria are an essential organelle in nearly every eukaryotic organism that perform vital functions such as ATP production, redox signaling, reactive oxygen species (ROS) homeostasis and regulation of programmed cell death. These activities are regulated by electrophysiological processes that occur in the inner mitochondrial membrane (IMM) and outer mitochondrial membrane (OMM) in response to metabolic demands, making them an important physiological marker for bioenergetic studies. Mitochondria dysfunction is an early pathological biomarker of complex diseases, such as diabetes, neurodegeneration, myopathy, cancer, and cardiovascular disease. Built atop a novel microfabrication strategy for 3D Microelectrode Arrays (MEAs), we demonstrate a 3D mitochondria biosensor capable of bimodal sensing of mitochondrial electrophysiology from the OMM and IMM using electrochemical impedance spectroscopy (EIS) and electrophysiology recordings. Data obtained through these measurements represent major functional outputs of cellular respiration, oxidative phosphorylation, and the regulation of ATP synthesis.

Completion Date

2024

Semester

Fall

Committee Chair

Rajaraman, Swaminathan

Degree

Master of Science (M.S.)

College

College of Medicine

Department

Burnett School of Biomedical Sciences

Degree Program

Biotechnology

Format

PDF

Identifier

DP0028997

Language

English

Release Date

12-15-2024

Access Status

Thesis

Campus Location

Orlando (Main) Campus

Accessibility Status

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