Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa
Abbreviated Journal Title
J. Antimicrob. Chemother.
CELL-ENVELOPE; Infectious Diseases; Microbiology; Pharmacology & Pharmacy
Aminoglycosides disrupt the outer membrane of Pseudomonas aeruginosa to facilitate access to their intracellular target. High-resolution X-ray micrography of live specimens is a relatively new technique. We used laser (nanosecond) plasma to image live cells of P. aeruginosa ATCC 27853. After exposure to 25 mg/L gentamicin for 15 min. we observed perturbation of the cell surface, membrane blebbings (370 nm and 273 nm diameter) away from the sell, formation of distinct channels (241 nm long) resulting from indentation and induction of cell elongation from 3-3.6 mu m (control) to 4.6-5.26 mu m (gentamicin-treated cells). These data illustrate the potential of high-resolution X-ray micrography for studying effects of drugs on live microbiological specimens.
Journal of Antimicrobial Chemotherapy
"Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa" (1998). Faculty Bibliography 1990s. 2412.