Title

Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa

Authors

Authors

J. M. Rajyaguru; D. S. Torres; E. Abel; M. C. Richardson;M. J. Muszynski

Comments

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Abbreviated Journal Title

J. Antimicrob. Chemother.

Keywords

CELL-ENVELOPE; Infectious Diseases; Microbiology; Pharmacology & Pharmacy

Abstract

Aminoglycosides disrupt the outer membrane of Pseudomonas aeruginosa to facilitate access to their intracellular target. High-resolution X-ray micrography of live specimens is a relatively new technique. We used laser (nanosecond) plasma to image live cells of P. aeruginosa ATCC 27853. After exposure to 25 mg/L gentamicin for 15 min. we observed perturbation of the cell surface, membrane blebbings (370 nm and 273 nm diameter) away from the sell, formation of distinct channels (241 nm long) resulting from indentation and induction of cell elongation from 3-3.6 mu m (control) to 4.6-5.26 mu m (gentamicin-treated cells). These data illustrate the potential of high-resolution X-ray micrography for studying effects of drugs on live microbiological specimens.

Journal Title

Journal of Antimicrobial Chemotherapy

Volume

41

Issue/Number

5

Publication Date

1-1-1998

Document Type

Article

DOI Link

http://dx.doi.org/10.1093/jac/41.5.557

Language

English

First Page

557

Last Page

561

WOS Identifier

WOS:000074481700007

ISSN

0305-7453

Recommended Citation

"Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa" (1998). Faculty Bibliography 1990s. 2412.
https://stars.library.ucf.edu/facultybib1990/2412