The role of human innate immune factors in nasal colonization by Staphylococcus aureus
Abbreviated Journal Title
Staphylococcus aureus; human nasal colonization; innate immunity; gene; polymorphism; MANNOSE-BINDING LECTIN; ANTIMICROBIAL PEPTIDES; GENE POLYMORPHISMS; EPITHELIAL-CELLS; DEFENSINS; INFECTIONS; CARRIAGE; SUSCEPTIBILITY; INFLAMMATION; ANTIBIOTICS; Immunology; Infectious Diseases; Microbiology
Slaphylococcus aureus colonization of the human nares predisposes to sometimes severe auto-infection. To investigate whether genetic polymorphism affects the S. aureus carriage status, sequence variation in alpha-defensin and beta-defensin, and mannose-binding lectin (MBL) genes were determined for a group of volunteers (n = 109) with known S. aureus nasal carriage status. DEFA113 expression was measured in a subset of the volunteers (n = 32). None of the single nucleotide polymorphisms studied could clearly distinguish the (non) carriage groups. S. aureus carriers differed from non-carriers in baseline level of HNP 1 - 3 peptide production (median: 218 versus 89 mu g/ml, P = 0.016). No association between HNPI-3 levels and the individual sequence polymorphisms was documented. The combined copy numbers of DEFAIIA3 genes ranged from 5 to 23 per diploid genome. A linear correlation between combined copy numbers and HNPI-3 peptide concentrations in nasal secretions of non-carriers was noted (r(2) = 0.8991). DEFA3 gene was absent in 25% of the individuals. MEL haplotype A was overrepresented in persistent S. aureus carriers (87% vs. 67%; P = 0.038). In conclusion, defensin gene polymorphism, both in sequence and in gene copy numbers, does not seem to be involved in S. aureus carriage predisposition. However, MBL haplotypes do so significantly. Baseline HNPI - 3 production is more the consequence of S. aureus colonization than a reason for the (non) carrier status. (c) 2007 Elsevier Masson SAS. All rights reserved.
Microbes and Infection
"The role of human innate immune factors in nasal colonization by Staphylococcus aureus" (2007). Faculty Bibliography 2000s. 7734.