Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1
Abbreviated Journal Title
Sensory neuron; Stem cell; Cell differentiation; Human; neural crest; stem cell; Neural progenitor; Schwann cell; DORSAL-ROOT GANGLIA; SERUM-FREE MEDIUM; SPINAL-CORD; EMBRYONIC; MOTONEURONS; ORGANOSILANE SURFACE; DOPAMINE NEURONS; DEFINED SYSTEM; IN-VITRO; DIFFERENTIATION; RAT; Engineering, Biomedical; Materials Science, Biomaterials
Although sensory neurons constitute a critical component for the proper function of the nervous system, the in vitro differentiation of functional sensory neurons from human stem cells has not yet been reported. This study presents the differentiation of sensory neurons (SNs) from a human neural progenitor cell line, hNP1, and their functional maturation in a defined, in vitro culture system without murine cell feeder layers. The SNs were characterized by immunocytochemistry and their functional maturation was evaluated by electrophysiology. Neural crest (NC) precursors, as one of the cellular derivatives in the differentiation culture, were isolated, propagated, and tested for their ability to generate sensory neurons. The hSC-derived SNs, as well as the NC precursors provide valuable tools for developing in vitro functional systems that model sensory neuron-related neural circuits and for designing therapeutic models for related diseases. (C) 2013 Elsevier Ltd. All rights reserved.
"Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1" (2013). Faculty Bibliography 2010s. 4064.