Title

From surface to intracellular non-invasive nanoscale study of living cells impairments

Authors

Authors

M. Ewald; L. Tetard; C. Elie-Caille; L. Nicod; A. Passian; E. Bourillot;E. Lesniewska

Comments

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Abbreviated Journal Title

Nanotechnology

Keywords

mode-synthesizing atomic force microscopy; subsurface imaging; living; cells; nanoscale resolution; keratinocytes; non-invasive; glyphosate; ATOMIC-FORCE MICROSCOPY; TAPPING-MODE; LIVE CELLS; HOLOGRAPHY; CANCER; HACAT; LINE; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied

Abstract

Among the enduring challenges in nanoscience, subsurface characterization of living cells holds major stakes. Developments in nanometrology for soft matter thriving on the sensitivity and high resolution benefits of atomic force microscopy have enabled detection of subsurface structures at the nanoscale. However, measurements in liquid environments remain complex, in particular in the subsurface domain. Here we introduce liquid-mode synthesizing atomic force microscopy (I-MSAFM) to study both the inner structures and the chemically induced intracellular impairments of living cells. Specifically, we visualize the intracellular stress effects of glyphosate on living keratinocytes skin cells. This new approach, I-MSAFM, for nanoscale imaging of living cell in their physiological environment or in presence of a chemical stress agent could resolve the loss of inner structures induced by glyphosate, the main component of a well-known pesticide (RoundUp (TM)). This firsthand ability to monitor the cell's inner response to external stimuli nondestructively and in liquid, has the potential to unveil critical nanoscale mechanisms of life science.

Journal Title

Nanotechnology

Volume

25

Issue/Number

29

Publication Date

1-1-2014

Document Type

Article

Language

English

First Page

8

WOS Identifier

WOS:000338716100001

ISSN

0957-4484

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