Title
Membrane Remodeling Induced by the Dynamin-Related Protein Drp1 Stimulates Bax Oligomerization
Abbreviated Journal Title
Cell
Keywords
CYTOCHROME-C RELEASE; BCL-2 FAMILY-MEMBERS; MITOCHONDRIAL; OUTER-MEMBRANE; CELL-DEATH; APOPTOTIC PROTEINS; FISSION; FUSION; PERMEABILIZATION; ACTIVATION; ASSOCIATION; Biochemistry & Molecular Biology; Cell Biology
Abstract
In response to many apoptotic stimuli, oligomerization of Bax is essential for mitochondrial outer membrane permeabilization and the ensuing release of cytochrome c. These events are accompanied by mitochondrial fission that appears to require Drp1, a large GTPase of the dynamin superfamily. Loss of Drp1 leads to decreased cytochrome c release by a mechanism that is poorly understood. Here we show that Drp1 stimulates tBid-induced Bax oligomerization and cytochrome c release by promoting tethering and hemifusion of membranes in vitro. This function of Drp1 is independent of its GTPase activity and relies on arginine 247 and the presence of cardiolipin in membranes. In cells, overexpression of Drp1 R247A/E delays Bax oligomerization and cell death. Our findings uncover a function of Drp1 and provide insight into the mechanism of Bax oligomerization.
Journal Title
Cell
Volume
142
Issue/Number
6
Publication Date
1-1-2010
Document Type
Article
Language
English
First Page
889
Last Page
901
WOS Identifier
ISSN
0092-8674
Recommended Citation
"Membrane Remodeling Induced by the Dynamin-Related Protein Drp1 Stimulates Bax Oligomerization" (2010). Faculty Bibliography 2010s. 553.
https://stars.library.ucf.edu/facultybib2010/553
Comments
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