Novel Hydrophilic Bis(1,2,3-triazolyl)fluorenyl Probe for In vitro Zinc Ion Sensing
Abbreviated Journal Title
ACS Appl. Mater. Interfaces
1, 2, 3-triazolyl-fluorene; two-photon microscopy bioimaging; fluorescent; probe; zinc ion sensing; metal sensing; binding constant; ABSORBING FLUORENE DERIVATIVES; ONE-PHOTON; PHOTOCHEMICAL PROPERTIES; 2-PHOTON MICROSCOPY; FLUORESCENT SENSOR; CLICK CHEMISTRY; CROSS-SECTION; DESIGN; PHOTOSTABILITY; EXCITATION; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
A hydrophillic bis(1,2,3-triazoly1)fluorene derivative was synthesized as a multi-photon absorbing zinc-ion sensing fluorescent probe. The fluorescent response was approximately five fold greater in presence of Zn(2+) resulting in a large binding constant (1 x 10(9)) for a 1/2 ligand to zinc complex. A four fold increase in the two-photon absorption cross section was achieved upon binding Zn(2+). In vitro two photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2(+) into HeLa cells and reversible Zn(2+) binding demonstrating the potential of this probe for zinc ion sensing.
Acs Applied Materials & Interfaces
"Novel Hydrophilic Bis(1,2,3-triazolyl)fluorenyl Probe for In vitro Zinc Ion Sensing" (2010). Faculty Bibliography 2010s. 592.