Title

Cis-regulatory analysis of the sea urchin pigment cell gene polyketide synthase

Authors

Authors

C. Calestani;D. J. Rogers

Comments

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Abbreviated Journal Title

Dev. Biol.

Keywords

Cis-regulation; Pigment cell; Sea urchin; Polyketide synthase; Glial; cells missing; Notch; Differentiation; Mesoderm; Transcriptional; regulation; Gene regulatory network; TRANSCRIPTION FACTOR; STRONGYLOCENTROTUS-PURPURATUS; MESODERM; SPECIFICATION; BETA-CATENIN; EMBRYO; EXPRESSION; NETWORK; DNA; BINDING; NOTCH; Developmental Biology

Abstract

The Strongylocentrotus purpuratus polyketide synthase gene (SpPks) encodes an enzyme required for the biosynthesis of the larval pigment echinochrome. SpPks is expressed exclusively in pigment cells and their precursors starting at blastula stage. The 7th-9th cleavage Delta-Notch signaling, required for pigment cell development, positively regulates SpPks. In previous studies, the transcription factors glial cell missing (SpGcm), SpGatae and kruppel-like (SpKrl/z13) have been shown to positively regulate SpPks. To uncover the structure of the Gene Regulatory Network (GRN) regulating the specification and differentiation processes of pigment cells, we experimentally analyzed the putative SpPks cis-regulatory region. We established that the -1.5 kb region is sufficient to recapitulate the correct spatial and temporal expression of SpPks. Predicted DNA-binding sites for SpGcm, SpGataE and SpKrl are located within this region. The mutagenesis of these DNA-binding sites indicated that SpGcm. SpGataE and SpKrl are direct positive regulators of SpPks. These results demonstrate that the sea urchin GRN for pigment cell development is quite shallow, which is typical of type I embryo development. (C) 2010 Elsevier Inc. All rights reserved.

Journal Title

Developmental Biology

Volume

340

Issue/Number

2

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

249

Last Page

255

WOS Identifier

WOS:000276942600010

ISSN

0012-1606

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